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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in lots of tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential position in endorsing mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence data is not really nevertheless accessible.
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Blocking peptides are peptides that bind especially for the concentrate on antibody and block antibody binding. These peptide generally has the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines additional feasible optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody on your own, you can see which staining is particular.
Focus on Facts Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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